Cell Culture and Murine Hepatocyte Isolation

Human fibrosarcoma HT1080 cells (KCLB no. 10121), human prostate adenocarcinoma PC-3 cells (KCLB no. 21435), and human gastric carcinoma AGS cells (KCLB no. 21739) were obtained from the Korean Cell Line Bank (Seoul, Korea) and cultured in RPMI 1640 (Cellgro, Manassas, VA, USA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (Cellgro) and penicillin (100 U/mL)/streptomycin (100 μg/mL) (Cellgro) at 37°C in a humidified 5% CO₂ incubator. Murine hepatocytes were isolated using a perfusion system with some modification and incubated as described previously [19 (link)].

Free full text: Click here

Kim A., Im M., Hwang Y.H., Yang H.J, & Ma J.Y. (2015). Jaeumganghwa-Tang Induces Apoptosis via the Mitochondrial Pathway and Lactobacillus Fermentation Enhances Its Anti-Cancer Activity in HT1080 Human Fibrosarcoma Cells. PLoS ONE, 10(5), e0127898.

Publication 2015

PC-3 cells RPMI 1640 heat-inactivated fetal bovine serum penicillin streptomycin

Adenocarcinoma Carcinoma Cell line Cells Fetal bovine serum Fibrosarcoma Gastric Hepatocytes Human Korean Murine Pc 3 cells Penicillin Perfusion Prostate Streptomycin

Corresponding Organization :

Other organizations : Korea Institute of Oriental Medicine

Top 5 similar protocols

Variable analysis

independent variables

Cell lines (human fibrosarcoma HT1080 cells, human prostate adenocarcinoma PC-3 cells, and human gastric carcinoma AGS cells)

dependent variables

Cell growth and behavior

control variables

Cell culture conditions (RPMI 1640 medium, 10% heat-inactivated fetal bovine serum, penicillin/streptomycin, 37°C, 5% CO2 incubator)
Murine hepatocytes (isolated using a perfusion system)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!