Lung Tissue Fixation and Transmission Electron Microscopy

Lungs were gravity inflation–fixed with 2% paraformaldehyde, 2% glutaraldehyde, and 0.1% calcium chloride in 0.1 M sodium cacodylate buffer, pH 7.2, followed by immersion fixation with fresh fixative at 4°C overnight. Lung lobes were cut into 1–2 mm blocks and processed for transmission electron microscopy as previously described (66 (link)). Images were digitally acquired by an H-7650 transmission electron microscope (Hitachi High Technologies) equipped with a CCD camera (Advanced Microscopy Techniques) at 80 kV.

Free full text: Click here

He H., Snowball J., Sun F., Na C.L, & Whitsett J.A. (2021). IGF1R controls mechanosignaling in myofibroblasts required for pulmonary alveologenesis. JCI Insight, 6(6), e144863.

Publication 2021

H-7650 transmission electron microscope CCD camera

Buffer Cacodylate Calcium Calcium chloride Chloride sodium Fixative Glutaraldehyde Gravity Immersion Lung Microscopy Paraformaldehyde Sodium Transmission electron microscope

Corresponding Organization :

Other organizations : Pulmonary Associates, Cincinnati Children's Hospital Medical Center

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Gravity inflation–fixed with 2% paraformaldehyde, 2% glutaraldehyde, and 0.1% calcium chloride in 0.1 M sodium cacodylate buffer, pH 7.2

dependent variables

Transmission electron microscopy images of lung lobes

control variables

Immersion fixation with fresh fixative at 4°C overnight
Lung lobes cut into 1–2 mm blocks

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!