Overexpression and Silencing of Key Proteins

Scrambled and pcDNA-3.1-14-3-3η-Flag were synthesized by Generay Biotech (Shanghai, China); while 14–3-3η- and NF-κB/p65 siRNA were purchased from Santa Cruz Biotechnology (http://datasheets.scbt.com/sc-43581.pdf, and https://datasheets.scbt.com/sc-29410.pdf) [12 (link), 16 (link)]. Cells were transiently transfected via lipofectamine 2000 reagent (Invitrogen, Carlsbad, USA), according to the manufacturer’s protocol (Note: plasmids used: 5 ng/ml. siRNAs used: 20 nM). After transfection, the cells were cultured in fresh medium supplemented with 10% FBS for another 24 h before being used for other experiments.

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Qiu Y., Dai Y., Zhang C., Yang Y., Jin M., Shan W., Shen J., Lu M., Tang Z., Ju L., Wang Y., Jiao R., Xia Y., Huang G., Yang L., Li Y., Zhang J., Wong V.K, & Jiang Z. (2018). Arsenic trioxide reverses the chemoresistance in hepatocellular carcinoma: a targeted intervention of 14–3-3η/NF-κB feedback loop. Journal of Experimental & Clinical Cancer Research : CR, 37, 321.

Publication 2018

NF-κB/p65 siRNA lipofectamine 2000 reagent

Cells Cultured medium Lipofectamine 2000 Nf κb p65 Plasmids Sirnas Transfection

Corresponding Organization :

Other organizations : Second Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Macau University of Science and Technology

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Variable analysis

independent variables

Scrambled
PcDNA-3.1-14-3-3η-Flag
14–3-3η- siRNA
NF-κB/p65 siRNA

dependent variables

Not explicitly mentioned

control variables

Cells were cultured in fresh medium supplemented with 10% FBS for another 24 h before being used for other experiments

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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