Lung Viral Titers and Antibody Response

Mice were sacrificed at 2 and 4 days p.i., lungs were collected and snap frozen for preparing the lung homogenates for viral titer measurement according to our previous publication⁶⁰. Mice were sacrificed at 15 days p.i., and whole blood was collected through the posterior vena cava vein. Serum was separated from whole blood by centrifugation (12,000 × g, 10 min at room temperature). The IAV-specific IgG2a and IgA antibodies in serum were determined using ELISA via serial dilution as described in our previous publication⁶⁰.

Free full text: Click here

Wang Q., Sundar I.K., Lucas J.H., Park J.G., Nogales A., Martinez-Sobrido L, & Rahman I. (2023). Circadian clock molecule REV-ERBα regulates lung fibrotic progression through collagen stabilization. Nature Communications, 14, 1295.

Publication 2023

Antibodies iga Blood Centrifugation Dilution Elisa Frozen Igg2a Lung Mice Posterior vena cava Serum Vein

Corresponding Organization :

Other organizations : University of Rochester Medical Center, University of Kansas Medical Center, Texas Biomedical Research Institute

Top 5 similar protocols

Variable analysis

independent variables

Time post-infection (p.i.): 2 days, 4 days, 15 days

dependent variables

Viral titer in lung homogenates
IAV-specific IgG2a and IgA antibody levels in serum

control variables

Mice were used as the experimental model

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!