Bioluminescent Pseudomonas aeruginosa Strain

Pseudomonas aeruginosa American Type Culture Collection (ATCC) 19660 (strain 180), an ExoU expression strain,^{20 (link)} was used as the representative causative pathogen. The strain was made bioluminescent by transfecting lux operon into the bacterial strain as described previously.^{21 (link)} This allowed noninvasive and real-time monitoring of bacterial bioluminescence, which is related to the corresponding colony-forming units (CFU) of bacteria, by using bioluminescence imaging.^{22 (link)} Bacteria were routinely grown in brain–heart infusion (BHI) medium (Fisher Scientific, Agawam, MA, USA) supplemented with 50 μg/mL kanamycin in an orbital incubator (37°C; 0.14g; New Brunswick Scientific, Edison, NJ, USA).

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Zhu H., Kochevar I.E., Behlau I., Zhao J., Wang F., Wang Y., Sun X., Hamblin M.R, & Dai T. (2017). Antimicrobial Blue Light Therapy for Infectious Keratitis: Ex Vivo and In Vivo Studies. Investigative Ophthalmology & Visual Science, 58(1), 586-593.

Publication 2017

BHI) medium

Bacteria Brain Heart Kanamycin Operon Pathogen Pseudomonas aeruginosa Strain

Corresponding Organization : Shanghai Jiao Tong University

Other organizations : Massachusetts General Hospital, Harvard University, Tufts University, Mount Auburn Hospital, Chinese PLA General Hospital

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Variable analysis

independent variables

Pseudomonas aeruginosa American Type Culture Collection (ATCC) 19660 (strain 180), an ExoU expression strain

dependent variables

Bacterial bioluminescence, which is related to the corresponding colony-forming units (CFU) of bacteria

control variables

Bacteria were routinely grown in brain–heart infusion (BHI) medium (Fisher Scientific, Agawam, MA, USA) supplemented with 50 μg/mL kanamycin in an orbital incubator (37°C; 0.14g; New Brunswick Scientific, Edison, NJ, USA)

controls

The strain was made bioluminescent by transfecting lux operon into the bacterial strain as described previously.

Annotations

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