Fluorometric PTP1B Activity Assay

The PTP1B activity was measured by using the fluorometric Protein tyrosine phosphatase activity assay kit (BioVision). The recombinant PTP1B and immunoprecipitated PTP1B were applied to the working solution by following the instruction and acquiring the fluorescence from (Ex/Em = 368/460 nm) with the Synergy HT multi-detection microplate reader (Bio-TeK, Winooski, VT, USA). The data were collected with kinetic mode with a 20 s period for 10 min for calculate the change in fluorescence with time.

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Lin Y.C., Zeng W.T, & Lee D.Y. (2023). H2S- and Redox-State-Mediated PTP1B S-Sulfhydration in Insulin Signaling. International Journal of Molecular Sciences, 24(3), 2898.

Publication 2023

Protein tyrosine phosphatase activity assay kit

Assay Fluorescence Fluorometric Kinetic Protein Ptp1b Tyrosine phosphatase activity

Corresponding Organization : China Medical University

Top 5 similar protocols

Variable analysis

independent variables

Recombinant PTP1B
Immunoprecipitated PTP1B

dependent variables

PTP1B activity

control variables

Fluorometric Protein tyrosine phosphatase activity assay kit (BioVision)
Synergy HT multi-detection microplate reader (Bio-TeK, Winooski, VT, USA)
Kinetic mode with a 20 s period for 10 min

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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