Polyphenols and amygdalin were analysed on a Shimadzu Nexera X2 UHPLC coupled with mass spectrometer LCMS 8040 (Shimadzu, Japan) as described in Ben-Othman et al. (2021) (link) with slight modifications. A reverse phase column ACE Excel 3 (C18, PFP, 100 × 2.1 mm; from ACE® Advanced Chromatography Technologies Ltd., Scotland) and pre-column (SecurityGuard ULTRA, C18; from Phenomenex, USA) were used at 40 °C for the separation of individual compounds. The flow rate of the mobile phase was 0.4 mL/min; the sample size was 1 μL. The mobile phase consisted of 1% formic acid in Milli-Q water (A) and 1% formic acid in methanol (B). Separation was carried out for 38 min under the following conditions: gradient 0–10 min, 10–25% B; 10–15 min, 25–35% B; 15–27 min, 35%–80% 27–30min 80-95 29–34 min isocratic 95% B, and re-equilibration of the system with 10% B 5 min.
Phenolic compounds were identified by comparing the retention times, UV spectra, and parent/daughter ion masses with those of the standard compounds described in Ben-Othman et al. (2021) (link).
Phenolic compounds were identified by comparing the retention times, UV spectra, and parent/daughter ion masses with those of the standard compounds described in Ben-Othman et al. (2021) (link).
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