MDMA (5 mg/kg), Ketanserin (5 mg/kg), SB242084 (3 mg/kg), Pindolol (0.3 mg/kg) and RU486 (7.5 mg, approximately 30 mg/kg) were purchased from Sigma-Aldrich Co. (Sigma-Aldrich, Israel). All drugs were dissolved in saline (0.9% NaCl) and injected intraperitoneally (i.p.) in a 250 µl volume. Based on the literature [32 (link)–34 (link)] and in considering our preliminary study which didn’t find any significantly differences between saline and MDMA 3 mg/kg in LMA and because of the possibility of neurotoxicity produced by higher dose of 10 mg/kg [35 (link)], the 5 mg/kg dose was chosen for this study. The doses of Ketanserin and SB242084 were selected based on Shinoda’s report [36 (link)]. The dose of Pindolol was selected based on Nash’s report [22 (link)]. The dose of RU486 was selected based on our previous study [37 (link)]. All pre-treatments were administered i.p. 60 min prior to MDMA injection.
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