HPLC-ESI-MS Analysis of Melanin-A

HPLC–ESI–MS analysis of MEL-A was performed as a previously described method (Wang et al. 2019 (link)) on a Waters Acquity UPLC H-Class/SQD II system (Waters Corp., Milford, MA, USA) with an ACQUITY UPLC BEH Shield RP18 (100 mm × 2.1 mm, 1.7 μm) column. The MS detection was operated in positive ion electrospray mode with an acquisition range of m/z 50–1000 and a scan rate of 0.5 spectra/s. The mass spectral ions were identified by the calculation of elemental composition according to available literature (Goossens et al. 2016 (link)).

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Yu G., Wang X., Zhang C., Chi Z., Chi Z, & Liu G. (2022). Efficient production of mannosylerythritol lipids by a marine yeast Moesziomyces aphidis XM01 and their application as self-assembly nanomicelles. Marine Life Science & Technology, 4(3), 373-383.

Publication 2022

Acquity UPLC H-Class/SQD II system ACQUITY UPLC BEH Shield RP18

Hplc Ions Rp18 Scan

Corresponding Organization :

Other organizations : Ocean University of China, Qingdao National Laboratory for Marine Science and Technology

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Variable analysis

independent variables

HPLC–ESI–MS analysis
Waters Acquity UPLC H-Class/SQD II system
ACQUITY UPLC BEH Shield RP18 (100 mm × 2.1 mm, 1.7 μm) column

dependent variables

MS detection operated in positive ion electrospray mode
Acquisition range of m/z 50–1000
Scan rate of 0.5 spectra/s
Mass spectral ions identified by calculation of elemental composition

control variables

Waters Acquity UPLC H-Class/SQD II system
ACQUITY UPLC BEH Shield RP18 (100 mm × 2.1 mm, 1.7 μm) column

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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