Quantifying SLC6A1 Protein Expression

Expression level of SLC6A1 protein in PCa cell lines with or without transfection were detected by Western blot analysis according to the protocol of our previous studies [19 (link)–21 (link)]. The used antibodies detail are: anti- SLC6A1rabbit monoclonal antibody (Cat No. bs-6617R, Bioss Co Ltd.,China), anti-β-actin rabbit monoclonal antibody (Cat No. BM0627, Boster, China), anti-GAPDH rabbit monoclonal antibody (Cat No. 10494–1-AP, Proteintech, USA).

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Chen C., Cai Z., Zhuo Y., Xi M., Lin Z., Jiang F., Liu Z., Wan Y., Zheng Y., Li J., Zhou X., Zhu J, & Zhong W. (2020). Overexpression of SLC6A1 associates with drug resistance and poor prognosis in prostate cancer. BMC Cancer, 20, 289.

Publication 2020

anti-β-actin rabbit monoclonal antibody anti-GAPDH rabbit monoclonal antibody

Actin Anti antibody Antibodies Cell lines Gapdh Monoclonal antibody Protein Rabbit Transfection Western blot analysis

Corresponding Organization : Guizhou Provincial People's Hospital

Other organizations : Southern Medical University, Nanfang Hospital, South China University of Technology, Guangzhou Medical University, Guangzhou First People's Hospital, Second Affiliated Hospital of Guangzhou Medical University

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Variable analysis

independent variables

Transfection status (with or without transfection)

dependent variables

Expression level of SLC6A1 protein in PCa cell lines

control variables

β-actin protein expression (as a loading control)
GAPDH protein expression (as a loading control)

controls

Positive control: PCa cell lines expressing SLC6A1 protein
Negative control: PCa cell lines without SLC6A1 protein expression

Annotations

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