Metabolic Profiling of Rats in Calo-Cages

All rats were individually housed in Calo-cages of the PhenoMaster system (TSE, Bad Homburg, Germany) at a normal room temperature (25 °C). The first 6 h of measurement were deleted for compensation of acclimatization bias [20 (link)]. For three consequent days, automatic measurement of volumes of respiratory oxygen (VO₂), volumes of carbon dioxide (VCO₂), and total energy expenditure (TEE) per hour per Kilogram (kg) of rat body weights per kg of lean body mass (0.75% of body weight) and per rat weights were done. Besides, the respiratory exchange ratio (RQ), automatic food intake (FI), and manual water intake were recorded [21 (link)]. During the 3-day stay in the PhenoMaster system, the light and dietary interventions were maintained according to the original group.

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Abulmeaty M.M., Almajwal A.M., Alnumair K.S., Razak S., Hasan M.M., Fawzy A., Farraj A.I., Abudawood M, & Aljuraiban G.S. (2021). Effect of Long-Term Continuous Light Exposure and Western Diet on Adropin Expression, Lipid Metabolism, and Energy Homeostasis in Rats. Biology, 10(5), 413.

Publication 2021

Calo-cages PhenoMaster system

Acclimatization Body Body weight Carbon dioxide Dietary interventions Energy expenditure Food intake Light Oxygen Respiratory Respiratory volumes measurement Water intake

Corresponding Organization : Zagazig University

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Variable analysis

independent variables

Light and dietary interventions

dependent variables

Volumes of respiratory oxygen (VO2)
Volumes of carbon dioxide (VCO2)
Total energy expenditure (TEE) per hour per Kilogram (kg) of rat body weights per kg of lean body mass (0.75% of body weight) and per rat weights
Respiratory exchange ratio (RQ)
Automatic food intake (FI)
Manual water intake

control variables

Rats were individually housed in Calo-cages of the PhenoMaster system at a normal room temperature (25 °C)
The first 6 h of measurement were deleted for compensation of acclimatization bias

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