Western blotting and immunofluorescent assays were performed according to corresponding standard methods [25 (link)]. The specific antibodies used were listed as follows: PTEN (Cell Signaling Technology (CST), 1:1000 dilution), GSK3β (CST, 1:800 dilution), Akt (CST, 1:800 dilution), phospho (p) Akt (Ser473) (CST, 1:500 dilution), pGSK3β (Ser9) (CST, 1:500 dilution), GAPDH (CST, 1:5000 dilution), β-catenin (Abcam, 1:50 dilution), and Alexa Fluor 594-conjugated goat anti-rabbit secondary antibody (1:200, 2 mg/mL, Invitrogen). Fluorescence images were captured by a laser scanning confocal microscope (Zeiss, Germany).
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