Animals and Ethical Approval In vivo imaging was performed on four Xenopus laevis tadpoles at developmental stage 37/38, based on Nieuwkoop and Faber 1956. All experimental procedures were approved by the University of Kent Animal Welfare and Ethical Review Body (AWERB; Institutional Ethics Reference Number: 0037-SK-17). Reporting of all in vivo experimental work conforms with the ARRIVE guidelines.
Xenopus laevis embryos were supplied by the European Xenopus Resource Center (EXRC; Portsmouth UK). The Xenopus embryos were kept in water at 20 C until they reached the developmental stage 37/3837 (link). Prior to OS-PAM imaging, animals were anesthetized in MS-222 solution (ethyl 3-aminobenzoate methane sulfonate, Sigma-Aldrich UK39 (link). Animals remained anesthetised in MS-222 solution for the entire duration of the imaging procedure (4.2 min). All methods employed were performed in accordance with guidelines and regulations as described in the research protocol approved by the University of Kent Animal Welfare and Ethical Review Body.
Xenopus laevis embryos were supplied by the European Xenopus Resource Center (EXRC; Portsmouth UK). The Xenopus embryos were kept in water at 20 C until they reached the developmental stage 37/3837 (link). Prior to OS-PAM imaging, animals were anesthetized in MS-222 solution (ethyl 3-aminobenzoate methane sulfonate, Sigma-Aldrich UK39 (link). Animals remained anesthetised in MS-222 solution for the entire duration of the imaging procedure (4.2 min). All methods employed were performed in accordance with guidelines and regulations as described in the research protocol approved by the University of Kent Animal Welfare and Ethical Review Body.
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