Gelatin-based Antiviral Assay Protocol

According to a report by Alarcón-Segovia et al.36 (link), 2.5 g gelatin from bovine skin (Sigma-Aldrich, USA) with glycerol and MilliQ water in a 12-well culture plate into a thickness of 1-2 mm were maturated 15 minutes as forming polymer. 5 μl of 100 μg/ml coffee leaf (Coffea Arabica) extract, 75% (weight/weight [w/w]) Ethanol, or H₂O (control) were applied separately on skin-like gelatin membranes at room temperature from 15 minutes to 1 hour and added into 2 μl pseudo-viral particles (MOI=0.2) for 2 hours. Subsequently, they were added with 50 μl of medium for the Vpp infection assay.

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Wu C.S., Chiang H.M., Chen Y., Chen C.Y., Chen H.F., Su W.C., Wang W.J., Chou Y.C., Chang W.C., Wang S.C, & Hung M.C. (2022). Prospects of Coffee Leaf against SARS-CoV-2 Infection. International Journal of Biological Sciences, 18(12), 4677-4689.

Publication 2022

gelatin from bovine skin

Assay Bovine Coffea arabica Coffee Ethanol Gelatin Glycerol Infection Leaf Membranes Polymer Skin Viral particles

Corresponding Organization : Asia University

Other organizations : Institute of Biomedical Sciences, Academia Sinica, Biomedical Translation Research Center

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Variable analysis

independent variables

Type of treatment applied to skin-like gelatin membranes (5 μl of 100 μg/ml coffee leaf (Coffea Arabica) extract, 75% (weight/weight [w/w]) Ethanol, or H2O (control))

dependent variables

Vpp infection assay (measured outcome)

control variables

Thickness of skin-like gelatin membranes (1-2 mm)
Maturation time of skin-like gelatin membranes (15 minutes)
Pseudo-viral particles added (2 μl, MOI=0.2)
Medium added after treatment (50 μl)

positive controls

H2O (control) treatment

negative controls

None specified

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