Murine Model of Induced Myopia

Before and after the myopia induction, the refraction and axial length of all eyes were measured using a refractometer (Steinberis Transfer Center, Tübingen, Germany) and spectral domain optical coherent tomography (SD-OCT, Envisu R4310, Leica, Wetzlar, Germany), respectively, under anesthesia by medetomidine (0.75 mg/kg, Sandoz K.K., Tokyo, Japan), midazolam (4 mg/kg, Domitor^®, Orion Corporation, Espoo, Finland), and butorphanol tartrate (5 mg/kg, Meiji Seika Pharma Co., Ltd., Tokyo, Japan) dissolved in normal saline (MMB). For the myopia induction group, a −30 D lens was fixed onto the right eye at postnatal three weeks old, and the mice were kept for three weeks. For the normal group, mice were prepared and kept without any special treatment. The induction of myopia and the ocular measurement were based on a previously reported experimental murine model of myopia [5 (link)]. Three mice were used for the myopia induction group and the normal group, respectively. In accordance with a previous report [36 (link)], the thickness of the retina and choroid was captured at points that were ±300 μm and ±400 μm from the optic nerve, respectively, and measured using the NIH ImageJ software.