Cell climbing was placed in the 24-well plate, and 2 × 105 cells were inoculated into the 24-well plate and cultured overnight. 2 hours before harvest, we added EdU solution (10 μM/ml) and incubated for 20 minutes. In accordance with the manufacturer's directions, EdU Imaging Kits (K1075, Apexbio) was used to stain the proliferative cells.
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