Fecal samples were prepared as previously described[22 ]. Briefly, 1g of fecal samples was diluted, mixed, and homogenized in 5mL of protease inhibitor cocktail (Sigma Aldrich, St. Louis, MO, United States; P83401)[22 ]. Blood was obtained from patients and healthy control subjects at 8 am and serums were separated, collected and stored at ﹣80 °C before use. Concentrations of tumor necrosis factor α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-17 (IL-17), and Gal-3 were measured in serum and fecal supernatants of UC patients by using commercially available ELISA tests, according to the manufacturer’s instructions.
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Cytokine Profiling in Ulcerative Colitis
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Other organizations : University of Kragujevac
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Variable analysis
independent variables
- None explicitly mentioned
- Concentrations of tumor necrosis factor α (TNF-α)
- Concentrations of interleukin-6 (IL-6)
- Concentrations of interleukin-10 (IL-10)
- Concentrations of interleukin-17 (IL-17)
- Concentrations of Gal-3
- Serum from healthy control subjects
- Fecal samples from healthy control subjects (not explicitly mentioned)
- Positive control: Serum from healthy control subjects
- Negative control: Not explicitly mentioned
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