Microangiography was performed following a previously published protocol (Arnold et al., 2022 (link); Shin et al., 2019 (link)). Embryos were anaesthetised with Tricane and injected with 1 nl of Qtracker™ 655 Vascular labels (Thermo Fisher Scientific) at 7 dpf in the LFL for valve leakage experiments and imaged on a Leica TCS SP8 DLS microscope at ∼5 min post-injection.
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