To ascertain cell viability in
the presence of the isolated alkaloids, the assessment was conducted
using the CCK-8 kit.16 (link) A 96-well microplate
was utilized to culture 3T3-L1 preadipocytes (1 × 103 cells per well). Following a 12 h-incubation period, the cells were
exposed to varying concentrations of alkaloids for 48 h. The experimentation
was carried out following the specified guidelines provided by the
manufacturer. Subsequently, the optical density of the cell cultures
at 450 nm was quantified using a Synergy H4 microplate reader, which
was manufactured by BioTek (Winooski, VT).
the presence of the isolated alkaloids, the assessment was conducted
using the CCK-8 kit.16 (link) A 96-well microplate
was utilized to culture 3T3-L1 preadipocytes (1 × 103 cells per well). Following a 12 h-incubation period, the cells were
exposed to varying concentrations of alkaloids for 48 h. The experimentation
was carried out following the specified guidelines provided by the
manufacturer. Subsequently, the optical density of the cell cultures
at 450 nm was quantified using a Synergy H4 microplate reader, which
was manufactured by BioTek (Winooski, VT).
