MCF7, T47D, and ZR75–1 parental (P) cells were cultured as previously described (4 (link)). The EndoR cell derivatives [resistant to long-term estrogen deprivation (EDR), to tamoxifen (TamR), and to fulvestrant (FulR)] were established as previously described (4 (link),5 (link)). To generate resistant derivatives to palbociclib (SelleckChem), MCF7 P, MCF7 EDR, T47D P, and T47D EDR cells were exposed to progressively increasing doses of palbociclib up to 1 μM. T47D P-LM and T47D P/PalboR-LM were cultured and developed as described previously (6 (link)). All cell lines were authenticated at the MD Anderson Characterized Cell Line Core Facility, and were tested to be mycoplasma-free by MycoAlert™ Mycoplasma Detection Kit (Lonza). Because T47D P-LM and T47D P/PalboR-LM were grown in a different media from MCF7 P, MCF7 EDR, T47D EDR and their PalboR derivatives, all parallel molecular analyses were done independently.
Protocol detail
Establishing Palbociclib-Resistant Breast Cancer Cell Lines
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Cell line
Cells
Derivatives
Endor
Estrogen
Fulvestrant
Mcf7
Mycoplasma
Palbociclib
Parental
Tamoxifen
Corresponding Organization : Baylor College of Medicine
Other organizations : University of Naples Federico II, Dana-Farber Cancer Institute, Harvard University, Hospital of Prato, Eli Lilly (United States), Vanderbilt University Medical Center, Memorial Sloan Kettering Cancer Center, University of California, Los Angeles
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Variable analysis
independent variables
- Exposure to progressively increasing doses of palbociclib up to 1 μM in MCF7 P, MCF7 EDR, T47D P, and T47D EDR cells
- Establishment of palbociclib-resistant (PalboR) derivatives from MCF7 and T47D parental (P) and endocrine-resistant (EDR) cell lines
- MCF7 P, MCF7 EDR, T47D P, and T47D EDR cells
- T47D P-LM and T47D P/PalboR-LM cell lines were cultured and developed separately from the other cell lines
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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