Tissue Homogenization and Antioxidant Analysis

Portions of liver and kidney tissue, 75–100 mg were homogenized in 1 mL of 50 mM phosphate buffer (pH 7.0) by ultrasonic homogenizer SONOPLUS Bandelin HD2070 (Bandelin, Germany) using the MS73 probe (Bandelin, Germany) with 10% power. Homogenates were centrifuged by Micro 200R centrifuge (Hettich, Germany) for 15 minutes at a speed of 10 000 × g at +4°C. The supernatant was used for the measurements of glutathione and lipid peroxidation level, as well as catalase (CAT) activity. All methods are described in previously published work by Brzovic Saric et al. [29 (link)].
All parameters normalized in relation to exact protein content.