The helix wheel structures were constructed by HeliQuest (http://heliquest.ipmc.cnrs.fr/ ). Bacteria preparation, MD simulations, antibacterial properties, toxicity assays, plasma stability, pharmacokinetic analysis, membrane permeabilization, killing kinetic assay, biofilm assays, and resistance were performed by using standard approaches. Statistical analysis was performed with GraphPad Prism 6 software or Discovery Studio 3.1. A detailed description of materials and methods and all of the necessary data can be found in the SI Appendix .
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Antimicrobial peptides: design and evaluation
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Corresponding Organization :
Other organizations : Kunming Institute of Zoology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Kunming Medical University, First Affiliated Hospital of Kunming Medical University, Chinese University of Hong Kong
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Variable analysis
independent variables
- Helix wheel structures constructed by HeliQuest
- Antibacterial properties
- Toxicity assays
- Plasma stability
- Pharmacokinetic analysis
- Membrane permeabilization
- Killing kinetic assay
- Biofilm assays
- Resistance
- Bacteria preparation
- MD simulations
- Standard approaches used for all experiments
- Positive controls
- Negative controls
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