Bioaerosol Collection and Imaging

The RASC is a purpose-built personal clean room equipped with a high efficiency bioaerosol collection system, which captures all exhaled particulate matter (bioaerosols) at 100–300 L/min in 15 mL of collection medium (sterilized phosphate-buffered saline supplemented with Polymyxin B, Amphotericin B, Nalidixic acid, Trimethoprim and Azilocillin (PANTA) [Becton Dickinson]). Bioaerosol samples were concentrated and stained with DMN-trehalose (Olilux Biosciences Inc.) overnight, as previously reported, before transfer to nanowell devices for imaging (Dinkele et al., 2021 (link)).

Free full text: Click here

Dinkele R., Gessner S., Patterson B., McKerry A., Hoosen Z., Vazi A., Seldon R., Koch A., Warner D.F, & Wood R. (2024). Persistent Mycobacterium tuberculosis bioaerosol release in a tuberculosis-endemic setting. medRxiv.

Publication Preprint 2024

PANTA

Corresponding Organization : University of Cape Town

Other organizations : Amsterdam Institute for Global Health and Development, Desmond Tutu HIV Foundation, Health Foundation

Top 5 similar protocols

Variable analysis

independent variables

Bioaerosol collection system setup

dependent variables

Captured exhaled particulate matter (bioaerosols)

control variables

Sterilized phosphate-buffered saline supplemented with Polymyxin B, Amphotericin B, Nalidixic acid, Trimethoprim and Azilocillin (PANTA)
Overnight staining with DMN-trehalose

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!