Neonatal Intraventricular Hemorrhage and Hydrocephalus
Corresponding Organization :
Other organizations : Washington University in St. Louis
Variable analysis
- Injection of artificial CSF (aCSF) or hemoglobin constituted in aCSF
- Development of ventriculomegaly by P7
- Anesthesia (isoflurane 2–3% induction and 1.5% maintenance)
- Stereotaxic frame
- Injection site (right lateral ventricle at coordinates 1.4 mm lateral, 0.5 mm anterior, and 2.0 mm deep)
- Injection volume (20 μL)
- Injection rate (8000 nL/min)
- Post-injection needle retention (5 min)
- Incision closure with 6–0 Ethilon suture
- Recovery and return to cage with dam for 72 h before CSF tracer injection at P7
- Positive control: Injection of aCSF
- Negative control: Not explicitly mentioned
Annotations
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