Samples from vaccinated participants were tested for antibodies against SARS-CoV-2 receptor-binding domain with the Access SARS-CoV-2 IgG assay (Beckman Coulter).11 (link),12 (link) Anti-N antibodies were tested with the Platelia SARS-CoV-2 Total Ab Assay (Bio-Rad) according to manufacturer instructions. The SARS-CoV-2 pseudovirus neutralization assay was performed as described previously7 (link) with the use of a green fluorescent protein reporter–based pseudotyped virus with a vesicular stomatitis virus backbone coated with SARS-CoV-2 S protein. The lower level of diagnostic detection for IgG is 0.62, and the lower level that is considered neutralizing is 16. Additional information about antibody testing is provided in Supplementary Methods Section S3.
Protocol detail
SARS-CoV-2 Antibody and Neutralization Assays
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Anti antibodies
Antibodies
Antibody
Assay
Backbone
Diagnostic
Green fluorescent protein
Igg receptor
Sars cov 2
Sars cov 2 s protein
Vesicular stomatitis virus
Virus
Corresponding Organization :
Other organizations : Sheba Medical Center, Tel Aviv University, Israel Ministry of Health
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Variable analysis
independent variables
- Vaccination status of participants
- Antibodies against SARS-CoV-2 receptor-binding domain measured with the Access SARS-CoV-2 IgG assay
- Anti-N antibodies measured with the Platelia SARS-CoV-2 Total Ab Assay
- SARS-CoV-2 pseudovirus neutralization assessed using a green fluorescent protein reporter–based pseudotyped virus with a vesicular stomatitis virus backbone coated with SARS-CoV-2 S protein
- Manufacturer instructions were followed for the Platelia SARS-CoV-2 Total Ab Assay (Bio-Rad)
- The SARS-CoV-2 pseudovirus neutralization assay was performed as described previously
- Not explicitly mentioned
- Not explicitly mentioned
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