Measuring Antioxidant Enzyme Activities

The activity of SOD and CAT enzymes was measured from the collected colon tissues using the commercially available kits (ab65354 and ab83464), respectively, from Abcam, Cambridge, UK. In short, the DC tissues from mice were weighed, homogenised, and centrifuged at 1100× g for 15 min at 4 °C. The levels of SOD and CAT were measured in duplicates, and the assays were performed according to the manufacturer’s protocol. The SOD activity is presented as the % inhibition of superoxide production by SOD. At the same time, CAT activity is presented in U/mg protein [65 (link),66 (link)].