PBMCs from each group were cultured and proliferated with RPMI-1640 culture medium containing 10% FBS (both from Thermo Fisher Scientific, Inc.) and 50 U/ml rIL-2 (Sigma-Aldrich; Merck KGaA) for 24 h in vitro. Then, the PBMCs were harvested and equally divided into three portions. One portion was defined as the untreated group (0 h) and collected directly for RNA and protein extraction. The other two portions were sequentially cultured with Rosuvastatin for another 12 and 24 h, respectively, and were defined as the 12 and 24-h groups. Rosuvastatin (Sigma-Aldrich; Merck KGaA) was dissolved in DMSO at a stock solution of 20 mM and stored in aliquots at −20°C as previously described (19 (link)).
Protocol detail
PBMC Stimulation and Rosuvastatin Treatment
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Corresponding Organization :
Other organizations : First Affiliated Hospital of Bengbu Medical College
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Variable analysis
independent variables
- Rosuvastatin treatment duration (0 h, 12 h, 24 h)
- RNA expression
- Protein expression
- RPMI-1640 culture medium containing 10% FBS
- 50 U/ml rIL-2
- In vitro cell culture conditions
- Untreated group (0 h) as a control group
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