RNA Extraction and qRT-PCR Analysis

Total RNA was isolated from cells using Qiagen RNeasy Plus Mini kit (Cat. #74134; GmbH) as used and reported in [16 (link)]. The qScript cDNA Synthesis Kit (Cat#95047-100, Quanta Bio, Beverly, MA, USA) was used to convert 1 µg mRNA into cDNA. qRT–PCR was performed using the PerfeCTa SYBR Green FastMix ROX (Cat #95073-012, Quanta Bio, Beverly, MA, USA), according to the manufacturer’s protocols (18). Expression levels of β-Actin were used as an internal control. Real-time analysis was performed with an AriaMx Real-Time PCR System (Agilent AriaMx Real-Time PCR System) using the primers (Table S2) ordered from Integrated DNA Technologies (IDT), Leuven, Belgium).

Free full text: Click here

Hezkiy E.E., Kumar S., Gahramanov V., Yaglom J., Hesin A., Jadhav S.S., Guzev E., Patel S., Avinery E., Firer M.A, & Sherman M.Y. (2022). Search for Synergistic Drug Combinations to Treat Chronic Lymphocytic Leukemia. Cells, 11(22), 3671.

Publication 2022

RNeasy Plus Mini kit qScript cDNA Synthesis Kit PerfeCTa SYBR Green FastMix ROX AriaMx Real-Time PCR System

Actin Cdna Cells Mrna Primers Sybr green Synthesis

Corresponding Organization : Ariel University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Expression levels of β-Actin

control variables

Total RNA was isolated from cells using Qiagen RNeasy Plus Mini kit (Cat. #74134; GmbH) as used and reported in [16 (link)]
The qScript cDNA Synthesis Kit (Cat#95047-100, Quanta Bio, Beverly, MA, USA) was used to convert 1 µg mRNA into cDNA
QRT–PCR was performed using the PerfeCTa SYBR Green FastMix ROX (Cat #95073-012, Quanta Bio, Beverly, MA, USA), according to the manufacturer's protocols (18)
Real-time analysis was performed with an AriaMx Real-Time PCR System (Agilent AriaMx Real-time PCR System) using the primers (Table S2) ordered from Integrated DNA Technologies (IDT), Leuven, Belgium

controls

Expression levels of β-Actin were used as an internal control

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!