Metabolic profiling of the methylene chloride-methanolic extract of the soft coral; Nephthea sp. and its derived fractions was carried out as described by Abdelmohsen et al.31 (link),51 (link) using an Acquity Ultra Performance Liquid Chromatography system connected to a Synapt G2 HDMS quadrupole time-of-flight hybrid mass spectrometer (Waters, Milford, USA). Chromatographic separation was carried out using a BEH C18 column (2.1 × 100 mm, 1.7 μm particle size; Waters, Milford, USA) accompanied with a guard column (2.1 × 5 mm, 1.7 μm particle size). The mobile phase used during the separation consisted of purified water (A) and acetonitrile (B) with 0.1% formic acid added to each solvent. A gradient elution started at a flow rate of 300 μL/min with 10% B linearly increased to 100% B within 30 min and remained isocratic for the next 5 min before linearly decreasing back to 10% B for the following 1 min. The volume injected was 2 μL and the column temperature was adjusted to 40°C. The obtained raw data were separated into positive and negative ionization mode using MSConvert software. Accordingly, the files were imported to the data mining software MZmine 2.10 for peak picking followed by deconvolution, deisotoping, alignment, and formula prediction. Dictionary of Natural Products (DNP) and Marinlit databases were used for the identification of compounds.53 ,54