Human tissue samples from cases and controls were harvested in the operating theater and immediately fixed with chilled buffer (50 mM sodium cacodylate [pH 7.4]) containing 2.5% glutaraldehyde and 2.0% paraformaldehyde and placed in 4°C overnight. The samples were then prepared as previsouly described9 (link). Samples analyzed with an FEI T-12 transmission electron microscope equipped with a side-mounted digital camera.
Protocol detail
Tissue Fixation and Electron Microscopy
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Buffer
Cacodylate
Glutaraldehyde
Human
Paraformaldehyde
Side
Sodium
Tissue
Transmission electron microscope
Corresponding Organization : Vanderbilt University
Other organizations : University of Virginia Health System, Mayo Clinic, Johns Hopkins University, New York University, VA Tennessee Valley Healthcare System
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Variable analysis
independent variables
- None explicitly mentioned
- Ultrastructural characteristics of human tissue samples
- Tissue samples from cases and controls
- Fixation with chilled buffer (50 mM sodium cacodylate [pH 7.4]) containing 2.5% glutaraldehyde and 2.0% paraformaldehyde
- Incubation at 4°C overnight
- Tissue samples from cases (presumably the condition of interest)
- Tissue samples from controls (presumably healthy or unaffected individuals)
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