5'RACE of Hepatocyte Transcripts

Total RNA from two different primary human hepatocyte cultures was used with 5′-RACE system 2.0 (Life Technologies, Eggenstein, Germany) as described previously (12 (link)).

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von Loeffelholz C., Lieske S., Neuschäfer-Rube F., Willmes D.M., Raschzok N., Sauer I.M., König J., Fromm M., Horn P., Chatzigeorgiou A., Pathe-Neuschäfer-Rube A., Jordan J., Pfeiffer A.F., Mingrone G., Bornstein S.R., Stroehle P., Harms C., Wunderlich F.T., Helfand S.L., Bernier M., de Cabo R., Shulman G.I., Chavakis T., Püschel G.P, & Birkenfeld A.L. (2017). The Human Longevity Gene Homolog INDY and Interleukin-6 Interact in Hepatic Lipid Metabolism. Hepatology (Baltimore, Md.), 66(2), 616-630.

Publication 2017

5′-RACE system 2.0

Cultures different Hepatocyte Human

Corresponding Organization : Paul Langerhans Institute Dresden

Other organizations : Friedrich Schiller University Jena, Jena University Hospital, University of Potsdam, Charité - Universitätsmedizin Berlin, Friedrich-Alexander-Universität Erlangen-Nürnberg, Deutsches Zentrum für Luft- und Raumfahrt e. V. (DLR), University of Cologne, Università Cattolica del Sacro Cuore, Max Planck Institute for Metabolism Research, Brown University, National Institute on Aging, National Institutes of Health, Howard Hughes Medical Institute, Yale University

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Variable analysis

independent variables

Primary human hepatocyte cultures

dependent variables

Results from 5'-RACE system 2.0 (Life Technologies, Eggenstein, Germany)

control variables

Experimental protocol used in a previous study (12)

controls

No specific positive or negative controls were mentioned.

Annotations

This protocol is too short. Unable to provide accurate annotations

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