Characterization of U-937 Cell Response to Electric Pulses

In most of the experiments we used U-937 (human monocyte lymphoma) cells. This cell line was chosen because the response of U-937 to electric pulses has been extensively investigated by several groups in the field including ours5 (link)6 (link)20 (link)24 (link)29 (link)30 (link). U-937 and HPAF-II (human pancreatic adenocarcinoma) cells were obtained from ATCC (Manassas, VA). U-937 grow in suspension and were cultured in RPMI-1640 medium (Sigma-Aldrich, St. Louis, MO). HPAF-II grow in a monolayer and were kept in EMEM medium (ATCC). Both growth media were supplemented with L-glutamine (ATCC), 10% (v/v) fetal bovine serum (Atlanta Biologicals, Norcross, GA), 100 U/ml penicillin and 0.1 mg/ml streptomycin (Mediatech Cellgro, Herdon, VA).

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Muratori C., Pakhomov A.G., Gianulis E.C., Jensen S.D, & Pakhomova O.N. (2016). The cytotoxic synergy of nanosecond electric pulses and low temperature leads to apoptosis. Scientific Reports, 6, 36835.

Publication 2016

HPAF-II RPMI-1640 medium EMEM medium L-glutamine fetal bovine serum

Adenocarcinoma Biologicals Cell line Cells Electric Fetal bovine serum Glutamine Growth media Human Lymphoma Monocyte Pancreatic Penicillin Pulses Streptomycin

Corresponding Organization :

Other organizations : Old Dominion University

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Variable analysis

independent variables

Electric pulses

dependent variables

Response of U-937 cells to electric pulses

control variables

U-937 (human monocyte lymphoma) cell line
HPAF-II (human pancreatic adenocarcinoma) cell line
RPMI-1640 medium (for U-937 cells)
EMEM medium (for HPAF-II cells)
L-glutamine supplementation
10% (v/v) fetal bovine serum
100 U/ml penicillin
0.1 mg/ml streptomycin

controls

Positive control: None specified
Negative control: None specified

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