Intracerebral Amyloid-β Infusion in Rats

Infusion of Aβ_(1–42) was performed as described earlier.^{23 (link)} Male Sprague-Dawley rats (300–380 g) were anesthetized by injecting a mixture of xylazine–ketamine and placing them on a stereotaxic frame. Injection was carried out by using a 27-gauge Hamilton syringe. A volume of 5 μl of 100 mM Aβ_1–42 in PBS was infused in the right cerebral cortex at stereotaxic co-ordinates from bregma: AP: −4.1, L: 2.5, DV: 1.3 mm, according to a previous report.^{51 (link)} An equal volume of Aβ_42–1 in PBS was injected in control animals. Animals were killed 21 days after injection. The brains were dissected out, following cardiac perfusion, and fixed in 4% PFA for 24 h. They were then incubated in a 30% sucrose solution for another 24 h before proceeding with cryosectioning (Cryotome; Thermo).