The ATAC-Seq libraries were prepared as previously described.40 (link) Briefly, 50,000 target cells were washed with PBS and then treated with lysis buffer, followed by labeling with the Nextera enzyme (15027865; Illumina). The labeled samples were immediately amplified by 9–10 cycles of polymerase chain reaction (PCR) with barcoded primers and sequenced with a HiSeq4000 instrument in a 150 bp/150 bp paired-end run or a NextSeq500 instrument in a 76 bp/76 bp paired-end run.
Chen X., Cao G., Wu J., Wang X., Pan Z., Gao J., Tian Q., Xu L., Li Z., Hao Y., Huang Q., Wang P., Xiao M., Xie L., Tang S., Liu Z., Hu L., Tang J., He R., Wang L., Zhou X., Wu Y., Chen M., Sun B., Zhu B., Huang J, & Ye L. (2019). The histone methyltransferase EZH2 primes the early differentiation of follicular helper T cells during acute viral infection. Cellular and Molecular Immunology, 17(3), 247-260.
Labeling with the Nextera enzyme (15027865; Illumina)
Number of PCR cycles (9–10 cycles)
dependent variables
Sequencing of the labeled samples with a HiSeq4000 instrument in a 150 bp/150 bp paired-end run or a NextSeq500 instrument in a 76 bp/76 bp paired-end run
control variables
Number of target cells (50,000)
Washing with PBS prior to treatment
controls
Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned
Annotations
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