Lineage Tracing in Mouse Embryos

CD1, C57BL/6 J wild-type, B6.SJL-Ptprca Pep3b/BoyJ, SCID-Beige mice (Charles River Laboratories), Jag1−/− (ref. 30 (link)), β-actin-GFP (ref. 31 (link)) and ROSA^eYFP (ref. 32 (link)) N1IP::Cre^LO (ref. 17 (link)) and N1IP::Cre^HI (ref. 19 (link)) strains were used. Animals were kept under pathogen-free conditions, and all procedures were approved by the Animal Care Committee of the Parc de Recerca Biomedica de Barcelona (regulation of Generalitat de Catalunya). Embryos were obtained from timed pregnant females and staged by somite counting: E10.5 (31–40 sp) E11.5 (43–48 sp). The detection of the vaginal plug was designated as day 0.5. Mice and embryos were genotyped using PCR when justified.

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Gama-Norton L., Ferrando E., Ruiz-Herguido C., Liu Z., Guiu J., Islam A.B., Lee S.U., Yan M., Guidos C.J., López-Bigas N., Maeda T., Espinosa L., Kopan R, & Bigas A. (2015). Notch signal strength controls cell fate in the haemogenic endothelium. Nature Communications, 6, 8510.

Publication 2015

B6.SJL-Ptprca Pep3b/BoyJ SCID-Beige mice

Actin Animal care committee Animals Embryos Mice Pathogen Pregnant females River Scid mice Somite Strains Vaginal

Corresponding Organization : University of Cincinnati

Other organizations : Pompeu Fabra University, University of Dhaka, Brigham and Women's Hospital, Harvard University, Hospital for Sick Children, Institució Catalana de Recerca i Estudis Avançats

Top 5 similar protocols

Variable analysis

independent variables

Jag1−/−
β-actin-GFP
ROSA^eYFP
N1IP::Cre^LO
N1IP::Cre^HI

dependent variables

Embryonic development stages: E10.5 (31–40 sp) and E11.5 (43–48 sp)

control variables

C57BL/6 J wild-type
B6.SJL-Ptprca Pep3b/BoyJ
SCID-Beige mice (Charles River Laboratories)

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