Tick Identification by MALDI-TOF MS

Based on the correlation of morphological and molecular results of tick identification, two to five specimens per species were used to assess MS spectra reproducibility from specimens of the same tick species, and the MS spectra specificity from specimens of distinct tick species. These analyses were performed with the average spectral profiles (MSP, Main Spectrum Profile) obtained from the four spots of each individual tick specimen using Flex analysis v.3.3 and ClinProTools 2.2 softwares (Bruker Daltonics). Tick species exhibiting reproducible and specific MS spectra were then included in-house MS spectra reference database. To upgrade the database, MSP reference spectra were created using spectra from at least 2 specimens per species of both genders by the automated function of the MALDI-Biotyper software v3.0. (Bruker Daltonics). MS spectra were created with an unbiased algorithm using information on the peak position, intensity and frequency [38 (link)]. The spectra files are available on request and transferable to any Bruker MALDI-TOF device.

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Diarra A.Z., Almeras L., Laroche M., Berenger J.M., Koné A.K., Bocoum Z., Dabo A., Doumbo O., Raoult D, & Parola P. (2017). Molecular and MALDI-TOF identification of ticks and tick-associated bacteria in Mali. PLoS Neglected Tropical Diseases, 11(7), e0005762.

Publication 2017

Flex analysis v.3.3 ClinProTools 2.2

Device Genders Maldi Spots Tick

Corresponding Organization :

Other organizations : Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Université des Sciences, des Techniques et des Technologies de Bamako, Mali-Folkecenter, Institut de Recherche pour le Développement, Inserm, Economic & Social Sciences, Health Systems & Medical Informatics

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Variable analysis

independent variables

Number of specimens per tick species used for MS spectra assessment (2 to 5 specimens)

dependent variables

MS spectra reproducibility from specimens of the same tick species
MS spectra specificity from specimens of distinct tick species

control variables

Automated function of the MALDI-Biotyper software v3.0 (Bruker Daltonics) used to create MSP reference spectra
Information on the peak position, intensity, and frequency used in the unbiased algorithm to create MS spectra

positive controls

Spectra from at least 2 specimens per species of both genders used to create MSP reference spectra

negative controls

No negative controls explicitly mentioned

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