Quantitative Protein Analysis in Membranes

Protein concentration was determined by the BCA protein assay [43 (link)]. Membranes were exposed to the following antibodies purchased from Cell Signaling Technology: Occludin (#91131), Claudin-2 (#48120), Cleaved-IL-1β (#63124), IL-18 (#57058), Caspase-1 (#2225), NLRP3 (#15101), ASC/TMS1 (#67824), AIM2(#63660), β-actin (#4970), Malt1(#2494), Bcl10(#4237), Nod1(#3545), Card9 (#12283), Ripk2 (#4142). Anti-ZO-1 antibody purchased from abcam (ab221547), Anti-NLRC4 antibody purchased from ECM Bioscience(#NP5381). Western blotting signals were quantified by a FluorChem densitometer (Alpha Innotech, San Leandro, CA).

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Xu Z., Li D., Qu W., Yin Y., Qiao S., Zhu Y., Shen S., Hou Y., Yang J, & Wang T. (2022). Card9 protects sepsis by regulating Ripk2-mediated activation of NLRP3 inflammasome in macrophages. Cell Death & Disease, 13(5), 502.

Publication 2022

Occludin Claudin-2 Cleaved-IL-1β IL-18 Caspase-1 NLRP3 ASC/TMS1 β-actin Malt1 Bcl10 Card9 Ripk2 FluorChem densitometer

Actin Anti antibody Antibodies Assay Bcl10 Card9 Caspase 1 Claudin 2 Il 18 Il 1β Malt1 Membranes Occludin Protein Ripk2

Corresponding Organization : Nanjing University

Top 5 similar protocols

Variable analysis

independent variables

Protein concentration determined by the BCA protein assay

dependent variables

Occludin
Claudin-2
Cleaved-IL-1β
IL-18
Caspase-1
NLRP3
ASC/TMS1
β-actin
Malt1
Bcl10
Card9
Ripk2
NLRC4

control variables

Exposure to antibodies purchased from Cell Signaling Technology and abcam
Western blotting signals quantified by a FluorChem densitometer (Alpha Innotech, San Leandro, CA)

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