Stress Vulnerability Assays in NPCs

Stress vulnerability assays were performed as previously described (Silva et al., 2016 (link)) (Figure 7B). NPCs were plated and differentiated in 96-well plate format, for eight weeks. Either QC-01–175, QC-03–075 or vehicle (DMSO) were added directly into the media (100 μL) to a final concentration of 5 μM, and incubated for 8 hr at 37°C. Then, each well was treated with either 10 μM of amyloid-beta(1-42) (Enzo Lifesciences, Farmingdale, NY), or vehicle alone, for an additional 16 hr incubation. At 24 hr, viability was measured with the Alamar Blue Cell viability reagent (Life Technologies), according to manufacturer instructions. Readings were done in the EnVision Multilabel Plate Reader (Perkin Elmer, Waltham, MA).