Arginine Metabolite Analysis Protocol

Benzoyl chloride (BCl), hydrochloride salts of unlabeled dimethylamine (D0-DMA), hexadeutero-dimethylamine (D6-DMA, declared as 99 at.% 2H), l-arginine, SDMA, ADMA, l-citrulline, and sodium tetraborate were procured from Sigma-Aldrich (Poznan, Poland). Isotope-labeled l-arginine:HCl (D7-arginine, 98%) and asymmetric dimethylarginine (2,3,3,4,4,5,5-D7-ADMA, 98%) were obtained from Cambridge Isotope Laboratories (Tewksbury, MA, USA). Methanol, acetonitrile, water, and formic acid were acquired from Merck Millipore (Warsaw, Poland), and leucine–enkephalin was obtained from Waters (Milford, MA, USA).

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Fleszar M.G., Wiśniewski J., Krzystek-Korpacka M., Misiak B., Frydecka D., Piechowicz J., Lorenc-Kukuła K, & Gamian A. (2018). Quantitative Analysis of l-Arginine, Dimethylated Arginine Derivatives, l-Citrulline, and Dimethylamine in Human Serum Using Liquid Chromatography–Mass Spectrometric Method. Chromatographia, 81(6), 911-921.

Publication 2018

l-arginine l-citrulline sodium tetraborate Isotope-labeled l-arginine:HCl (D7-arginine, asymmetric dimethylarginine Methanol acetonitrile formic acid leucine–enkephalin

Acetonitrile Arginine Asymmetric dimethylarginine Benzoyl chloride Dimethylamine Dimethylamine hydrochloride Formic acid Isotope L citrulline Leucine enkephalin Methanol Salts Sodium tetraborate

Corresponding Organization :

Other organizations : Wroclaw Medical University, The University of Texas at Arlington, Wroclaw Research Centre EIT+ (Poland)

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Protocol cited in 4 other protocols

Variable analysis

independent variables

Benzoyl chloride (BCl)
Hydrochloride salts of unlabeled dimethylamine (D0-DMA)
Hexadeutero-dimethylamine (D6-DMA, declared as 99 at.% 2H)
L-arginine
L-citrulline
Sodium tetraborate

dependent variables

Unspecified

control variables

Methanol
Acetonitrile
Water
Formic acid
Leucine–enkephalin

positive controls

Isotope-labeled L-arginine:HCl (D7-arginine, 98%)
Asymmetric dimethylarginine (2,3,3,4,4,5,5-D7-ADMA, 98%)

negative controls

None specified

Annotations

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