Cells were cultured in serum free medium (SFM) as previously reported to enrich CSCs from HCT116 and HT29[24 (link)]. The SFM consisted of DMEM/F12 medium (Life Technologies, United States) supplemented with 20 ng/mL epidermal growth factor (EGF, Invitrogen, United States), 10 ng/mL basic fibroblast growth factor (bFGF, Invitrogen, United States), 2% B27 supplement (Life Technologies, USA) in 6-well ultra-low-adherent plates (Cat#3471, Corning, NY, United States). Culture medium was replaced twice a week, and CSCs were passaged every six to seven days using TrypLE (Gibco, NY, United States).
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Enrichment of Cancer Stem Cells
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Corresponding Organization :
Other organizations : Chengdu University of Traditional Chinese Medicine, Liaoning University of Traditional Chinese Medicine, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine
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Variable analysis
independent variables
- Serum free medium (SFM)
- Enrichment of cancer stem cells (CSCs) from HCT116 and HT29 cell lines
- DMEM/F12 medium (Life Technologies, United States)
- 20 ng/mL epidermal growth factor (EGF, Invitrogen, United States)
- 10 ng/mL basic fibroblast growth factor (bFGF, Invitrogen, United States)
- 2% B27 supplement (Life Technologies, USA)
- 6-well ultra-low-adherent plates (Cat#3471, Corning, NY, United States)
- Replacement of culture medium twice a week
- Passaging of CSCs every six to seven days using TrypLE (Gibco, NY, United States)
- No positive or negative controls were explicitly mentioned in the provided information.
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