The cDNA was amplified, labeled, and hybridized to 60 K Agilent 60-mer oligo microarrays following the manufacturer's instructions. The Low Input Quick Amp Labeling Kit was used as the labeling reagent, with SurePrint G3 Human Gene Expression Microarray 8×60K as the microarray. All hybridized microarray slides were scanned with an Agilent scanner. Both the relative hybridization intensities and background hybridization values were calculated using Agilent Feature Extraction Software (9.5.1.1).
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Agilent 60-mer oligo microarray protocol
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Variable analysis
independent variables
- None explicitly mentioned
- Relative hybridization intensities
- Background hybridization values
- Agilent 60-mer oligo microarrays
- Low Input Quick Amp Labeling Kit
- SurePrint G3 Human Gene Expression Microarray 8×60K
- Agilent scanner
- Positive controls: Not explicitly mentioned
- Negative controls: Not explicitly mentioned
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