For detection of ROS cells were incubated with dihydrorhodamine 123 (DHR 123, Molecular Probes, 1 µg/ml, ChemCruz sc-203027) in DMEM without phenol red. After incubation for 15 min at 37 °C, ROS-induced fluorescence was measured on a Tecan microplate reader (excitation 488 nm, emission 530 nm).
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