Expression of BDNF and TrkB was determined by western blot analysis, as described previously [6 (link),20 (link)]. Hippocampal tissues were homogenized in lysis buffer. Protein (30 μg) was separated on sodium dodecyl sulfate-polyacrylamide gels, and then transferred onto a nitrocellulose membrane. Rabbit anti-BDNF (1:1,000; Santa Cruz Biotechnology), rabbit anti-TrkB (1:1,000; Santa Cruz Biotechnology), and mouse anti-β-actin (1:1,000; Santa Cruz Biotechnology) antibodies were used as primary antibodies. Horseradish peroxidase-conjugated antimouse antibody for β-actin and antirabbit antibody for BDNF and TrkB were used as secondary antibodies.
Protocol detail
Western Blot Analysis of BDNF and TrkB
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Actin
Antibodies
Antibody
Buffer
Horseradish peroxidase
Membrane
Mouse
Nitrocellulose
Polyacrylamide gels
Protein
Rabbit
Sodium dodecyl sulfate
Tissues
Trkb
Western blot analysis
Corresponding Organization :
Other organizations : Tongmyong University, Kyung Hee University, Korea University
Top 5 similar protocols
Variable analysis
independent variables
- None explicitly mentioned
- Expression of BDNF
- Expression of TrkB
- Hippocampal tissues
- Lysis buffer
- Protein (30 μg)
- Sodium dodecyl sulfate-polyacrylamide gels
- Nitrocellulose membrane
- Rabbit anti-BDNF (1:1,000; Santa Cruz Biotechnology) primary antibody
- Rabbit anti-TrkB (1:1,000; Santa Cruz Biotechnology) primary antibody
- Mouse anti-β-actin (1:1,000; Santa Cruz Biotechnology) primary antibody
- Horseradish peroxidase-conjugated antimouse antibody for β-actin secondary antibody
- Horseradish peroxidase-conjugated antirabbit antibody for BDNF and TrkB secondary antibodies
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!