Quantifying Kidney Damage Biomarkers
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Corresponding Organization :
Other organizations : Yale University, University of Miami, University of California, San Diego
Protocol cited in 1 other protocol
Variable analysis
- Experimental conditions
- Urinary albumin
- Plasma and 24-h urine creatinine
- Glomerular area
- Percentage of glomeruli per section containing mesangiolysis or nodules
- Semiquantitative pathology score (glomerular nodules, mesangiolysis, mesangial sclerosis, and interstitial fibrosis)
- Techniques used to measure urinary albumin (mouse albumin ELISA, SDS-PAGE/Coomassie blue staining)
- Techniques used to measure plasma and 24-h urine creatinine (high-performance liquid chromatography)
- Techniques used for transmission electron microscopy (TEM)
- Techniques used to measure glomerular area (ImageJ software)
- Techniques used for histological examination (periodic acid Schiff staining, blinded assessment by a renal pathologist)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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