Urinary albumin was measured using mouse albumin ELISA (Bethyl Laboratories) and SDS-PAGE/Coomassie blue staining, as described elsewhere (16 (link)). Plasma and 24-h urine creatinine were measured by high-performance liquid chromatography (16 (link)). Transmission electron microscopy (TEM) was performed using standard techniques (16 (link)). Glomerular area was measured using ImageJ software (National Institutes of Health, Bethesda, MD; http://rsb.info.nih.gov/ij/ ) in 34 ± 2.1 glomeruli/kidney from 4 mice per experimental condition. A renal pathologist (G.M.) examined in a blinded fashion kidney specimens stained with periodic acid Schiff and assigned a semiquantitative pathology score based on the percentage of the area (0 = none; 1 = 1–25%; 2 = 26–50%; 3 = 51–75%; 4 = 76–100%) with the following features: glomerular nodules, mesangiolysis, mesangial sclerosis, and interstitial fibrosis (24 (link)). The percentage of glomeruli per section containing mesangiolysis or nodules was calculated (28 (link)).
Protocol detail
Quantifying Kidney Damage Biomarkers
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Albumin
Coomassie blue
Creatinine
Elisa
Fibrosis
Glomeruli
High performance liquid chromatography
Kidney
Mesangial sclerosis
Mice
Pathologist
Periodic acid
Plasma
Sds page
Transmission electron microscopy
Urine
Corresponding Organization :
Other organizations : Yale University, University of Miami, University of California, San Diego
Top 5 similar protocols
Protocol cited in 1 other protocol
Variable analysis
independent variables
- Experimental conditions
- Urinary albumin
- Plasma and 24-h urine creatinine
- Glomerular area
- Percentage of glomeruli per section containing mesangiolysis or nodules
- Semiquantitative pathology score (glomerular nodules, mesangiolysis, mesangial sclerosis, and interstitial fibrosis)
- Techniques used to measure urinary albumin (mouse albumin ELISA, SDS-PAGE/Coomassie blue staining)
- Techniques used to measure plasma and 24-h urine creatinine (high-performance liquid chromatography)
- Techniques used for transmission electron microscopy (TEM)
- Techniques used to measure glomerular area (ImageJ software)
- Techniques used for histological examination (periodic acid Schiff staining, blinded assessment by a renal pathologist)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!