Immunohistochemical Analysis of Transgene Expression

The rostral-caudal extent of transgene expression was assessed by immunohistochemistry. Brain sections were immunostained as previously described (Guggenhuber et al., 2010 (link)) using a rabbit anti-HA primary antibody (Santa Cruz Biotechnology, Santa Cruz, CA; 1:1000). For colocalization studies, rabbit anti-MAGL (generous gift from Ken Mackie, 1:200), guinea pig anti-VGluT1 (Millipore, 1:500), and rabbit anti-CB1R (Frontier Institute, Hokkaido, Japan, 1:500) primary antibodies were used.

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Guggenhuber S., Romo-Parra H., Bindila L., Leschik J., Lomazzo E., Remmers F., Zimmermann T., Lerner R., Klugmann M., Pape H.C, & Lutz B. (2015). Impaired 2-AG Signaling in Hippocampal Glutamatergic Neurons: Aggravation of Anxiety-Like Behavior and Unaltered Seizure Susceptibility. International Journal of Neuropsychopharmacology, 19(2), pyv091.

Publication 2015

anti-VGluT1

Anti antibody Antibodies Brain Guinea pig Immunohistochemistry Rabbit Transgene

Corresponding Organization :

Other organizations : Johannes Gutenberg University Mainz, UNSW Sydney, University Medical Center of the Johannes Gutenberg University Mainz

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Variable analysis

independent variables

Immunostaining with a rabbit anti-HA primary antibody (Santa Cruz Biotechnology, Santa Cruz, CA; 1:1000)

dependent variables

Rostral-caudal extent of transgene expression

control variables

Brain sections
Immunostaining protocol as previously described (Guggenhuber et al., 2010)

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

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