Lineage Tracing with Rosa Reporter Mice

For lineage tracing, Rosa^RFP reporter mice (https://www.jax.org/strain/007908) were used [43 (link)] and mated to Six3 HET and GnRH^cre mice to create the Six3^HET:Rosa^RFP:GnRH^cre line. IHC was performed with the anti-RFP antibody (1:1000, Abcam, catalog number: ab62341, RRID: AB_945213).

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Pandolfi E.C., Hoffmann H.M., Schoeller E.L., Gorman M.R, & Mellon P.L. (2018). Haploinsufficiency of SIX3 Abolishes Male Reproductive Behavior through Disrupted Olfactory Development, and Impairs Female Fertility through Disrupted GnRH Neuron Migration. Molecular neurobiology, 55(11), 8709-8727.

Publication 2018

anti-RFP antibody ab62341

Anti antibody Mice Strain

Corresponding Organization :

Other organizations : University of California, San Diego

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Variable analysis

independent variables

Mating of Rosa^RFP reporter mice with Six3 HET and GnRH^cre mice to create the Six3^HET:Rosa^RFP:GnRH^cre line

dependent variables

Immunohistochemistry (IHC) performed with the anti-RFP antibody

control variables

Anti-RFP antibody used at a concentration of 1:1000

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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