Proximity ligation assay (PLA) was performed using the anti-Mouse MINUS probe, anti-Rabbit PLUS probe and the orange detection reagent (Sigma-Aldrich) as previously described(53 (link)). Actin was visualized using actistain 670 and imaged on a Leica scanning confocal (SP5) with a HCX Plan Apochromat 63×/1.40–0.60 NA oil λ blue objective. The total number of distinct PLA spots per cell were quantified using the threshold function of ImageJ.
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Proximity Ligation Assay for Protein Interactions
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Other organizations : SUNY Upstate Medical University
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Variable analysis
independent variables
- Anti-Mouse MINUS probe
- Anti-Rabbit PLUS probe
- Orange detection reagent (Sigma-Aldrich)
- Total number of distinct PLA spots per cell
- Actin was visualized using actistain 670
- Leica scanning confocal (SP5) with a HCX Plan Apochromat 63×/1.40–0.60 NA oil λ blue objective
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