After stratification of the seeds at 2°C for 5 days or vernalization at 2°C for 6 weeks on wet filter paper and in darkness, plants of Arabidopsis (Columbia) were grown in 8-h SDs or 16-h LDs. The photon flux density was 120 (HL) or 50 μmol.m-2.s-1 (LL) PAR (Very High Output fluorescent tubes; Sylvania, Zaventem, Belgium) and the temperature was 20°C. Relative humidity – which is critical for the success of germination and seedling establishment – was set optimally at 70%.
Protocol detail
Optimizing Arabidopsis Growth Conditions
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Other organizations : University of Liège
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Variable analysis
independent variables
- Duration of vernalization (2°C for 5 days or 6 weeks)
- Photoperiod (8-h short days or 16-h long days)
- Plant growth and development
- Temperature (20°C)
- Relative humidity (70%)
- Light intensity (120 μmol.m^-2.s^-1 or 50 μmol.m^-2.s^-1)
- Arabidopsis plant line (Columbia)
- Negative control: Not mentioned
- Positive control: Not mentioned
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