Silencing DNA repair genes in cells

The control siRNA (5’-UAAUGUAUUGGAACGCAUA-3’), BLM siRNA (5’-CCGAAUCUCAAUGUACAUAGA-3’)65 (link), RECQ5 siRNA (5’-CAGGAGGCUGAUAAAGGGUUA-3’)65 (link), TREX1 siRNA (5’-CCAAGACCAUCUGCUGUCA-3’)66 (link), CTIP siRNA (5’-GCUAAAACAGGAACGAAUC-3’)67 (link), GEN1 siRNA (5’-GUAAAGACCUGCAAUGUUA-3’) were purchased from Eurofins. The MUS81 siRNA (5’-CAGCCCUGGUGGAUCGAUA-3’ and 5’-CAUUAAGUGUGGGCGUCUA-3’)68 (link), SLX1 siRNA (5’-UGGACAGACCUGCUGGAGAUU-3’)69 (link), ERCC1 siRNA (SMARTpool ON-TARGET plus L-006311), MRE11 siRNA (SMARTpool ON-TARGET plus L-009271), EXO1 siRNA (SMARTpool ON-TARGET plus L-013120), DNA2 siRNA (SMARTpool ON-TARGET L-026431), RECQ1 siRNA (SMARTpool ON-TARGET plus L-013597), RECQ4 siRNA (SMARTpool ON-TARGET plus L-010559), WRN siRNA (SMARTpool ON-TARGET plus L-010378), BRCA2 siRNA (SMARTpool ON-TARGET plus L-003462) and RAD51 siRNA (SMARTpool ON-TARGET plus L-003530) were purchased from Dharmacon. Cells were seeded one day before siRNA treatment and transfected with 25 nM of siRNA using Lipofectamine RNAiMAX (Life Technologies).

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Chan Y.W., Fugger K, & West S.C. (2017). Unresolved recombination intermediates lead to ultra-fine anaphase bridges, chromosome breaks and aberrations. Nature cell biology, 20(1), 92-103.

Publication 2017

control siRNA BLM siRNA RECQ5 siRNA TREX1 siRNA CTIP siRNA GEN1 siRNA MUS81 siRNA SLX1 siRNA ERCC1 siRNA MRE11 siRNA EXO1 siRNA DNA2 siRNA RECQ1 siRNA RECQ4 siRNA WRN siRNA BRCA2 siRNA RAD51 siRNA Lipofectamine RNAiMAX

Brca2 Cells Ctip Dna2 Exo1 Gen1 Lipofectamine Recq1 Recq4 Recq5 Sirna Trex1

Corresponding Organization :

Other organizations : The Francis Crick Institute

Top 5 similar protocols

Variable analysis

independent variables

Control siRNA (5'-UAAUGUAUUGGAACGCAUA-3')
BLM siRNA (5'-CCGAAUCUCAAUGUACAUAGA-3')
RECQ5 siRNA (5'-CAGGAGGCUGAUAAAGGGUUA-3')
TREX1 siRNA (5'-CCAAGACCAUCUGCUGUCA-3')
CTIP siRNA (5'-GCUAAAACAGGAACGAAUC-3')
GEN1 siRNA (5'-GUAAAGACCUGCAAUGUUA-3')
MUS81 siRNA (5'-CAGCCCUGGUGGAUCGAUA-3' and 5'-CAUUAAGUGUGGGCGUCUA-3')
SLX1 siRNA (5'-UGGACAGACCUGCUGGAGAUU-3')
ERCC1 siRNA (SMARTpool ON-TARGET plus L-006311)
MRE11 siRNA (SMARTpool ON-TARGET plus L-009271)
EXO1 siRNA (SMARTpool ON-TARGET plus L-013120)
DNA2 siRNA (SMARTpool ON-TARGET L-026431)
RECQ1 siRNA (SMARTpool ON-TARGET plus L-013597)
RECQ4 siRNA (SMARTpool ON-TARGET plus L-010559)
WRN siRNA (SMARTpool ON-TARGET plus L-010378)
BRCA2 siRNA (SMARTpool ON-TARGET plus L-003462)
RAD51 siRNA (SMARTpool ON-TARGET plus L-003530)

dependent variables

Not explicitly mentioned

control variables

Lipofectamine RNAiMAX (Life Technologies)

Annotations

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