Histochemical and Morphological Evaluation of Tissue Samples

A morphological and a histochemical stains were used to process the slides [4 (link), 6 (link)]. Slices were then rehydrated using progressive alcohol solutions (from 100 to 30% ethanol), to distilled water after being deparaffinized in xylene [17 (link)],G. [75 (link)], subsequently, they were stained using Mallory trichrome and AB/PAS [3 (link)]. After staining slides were mounted using Eukitt (BioOptica Milano S.p.A, Milan, Italy, Europe). Data on the stains used in this study are included in Table 1.Table 1

Stains used in this study

Stain name	Type	Catalogue number	Supplier
Mallory trichrome	Morphological	04–020,802	BioOptica Milano S.p.A, Milan, Italy, Europe
Alcian blue/Periodic Acid Schiff (AB/PAS)	Histochemical	04–163,802	BioOptica Milano S.p.A, Milan, Italy, Europe

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Alesci A., Capillo G., Fumia A., Albano M., Messina E., Spanò N., Pergolizzi S, & Lauriano E.R. (2023). Coelomocytes of the Oligochaeta earthworm Lumbricus terrestris (Linnaeus, 1758) as evolutionary key of defense: a morphological study. Zoological Letters, 9, 5.

Publication 2023

Eukitt

Ethanol Periodic acid Stains Xylene

Corresponding Organization : University of Messina

Other organizations : National Research Council

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Variable analysis

independent variables

Morphological stain
Histochemical stain

dependent variables

Tissue morphology and histochemistry

control variables

Deparaffinization and rehydration process using progressive alcohol solutions
Staining with Mallory trichrome and AB/PAS
Mounting using Eukitt

controls

No positive or negative controls were explicitly mentioned.

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